It's too bad most business signs are just too big to put in some sort of museum. Tallahassee's had some great ones. The old Northwood Mall triangle/arrow. The neon Prince Murat motel sign, which so often seemed to advertise the Prince Rat. The Office Lounge sign, which actually looks modern-retro with its martini glass shape. Both the State Theatre and Florida Theatre signs, classic art deco. The Firestone "shield" sign on North Monroe that was recently replaced with a much less interesting piece (although the old signs are still used in several places on the building).
If you've got pictures of any classics, show 'em off. If I missed your favorites, let me know.